The Antibacterial And The Surface Activity Properties Of The Incured CSx And CSy Compounds Were Psychoanalyzed Against Listeria Monocytogenes, Staphylococcus Aureus, E

coli and Salmonella bacteria using the well diffusion method and the Zeiss microscope.Chitosan reenforced with Kenaf Nanocrystalline Cellulose as an Effective Carrier for the Delivery of Platelet Lysate in the Acceleration of Wound Healing.The clinical use of platelet lysate (PL) in the treatment of injurys is limited by its rapid degradation by proteases at the tissue site. This research aims to develop a chitosan (CS) and kenaf nanocrystalline cellulose (NCC) hydrogel composite, which intend to stabilize PL and control its release onto the wound site for prolonged action.  Clinical Nutrition  was synthesised from raw kenaf bast roughages and incorporated into the CS hydrogel. The physicochemical dimensions, in vitro cytocompatibility, cell proliferation, wound scratch assay, PL release, and CS stabilizing effect of the hydrogel complexs were psychoanalyzed.

The study of tumefying ratio (>1000%) and moisture loss (60-90%) indicated the excellent water retention capacity of the CS-NCC-PL hydrogels as likened with the commercial product. In vitro release PL study (flux = 0 mg/cm(2)/h) bespeaked that NCC act as a nanofiller and leaved the nourished release of PL equated with the CS hydrogel alone. The CS also showed the protective effect of growth factor (GF) present in PL, thereby boosting fast wound healing via the formulation. The CS-NCC hydrogels also augmented fibroblast proliferation in vitro and enhanced wound closings over 72 h.  Selenium  furnishs a new insight on CS with renewable source kenaf NCC as a nanofiller as a potential autologous PL wound therapy.Synthesis, characterization and application of chitosan-N-(4-hydroxyphenyl)-methacrylamide derivative as a drug and gene carrier.The aim of this study was to develop a green method to fabricate a novel CS qualifyed N-(4-hydroxyphenyl)- methacrylamide conjugate (CSNHMA) and to evaluate its biomedical potential.

CSNHMA has been maked by a simple method via aza Michael addition reaction between CS and N- (4-hydroxyphenyl)-methacrylamide (NHMA) in ethanol. Its structural and morphological props were characterized by various analysis techniques. The obtained results confirmed that a highly porous network structure of CSNHMA was successfully synthesised via aza Michael addition reaction it was examined as a drug and gene carrier. CSNHMA/pGL3 registered an enhanced buffering capacity due to the presence of NHMA moiety leading to higher transfection efficiency in all cancer cells (A549, HeLa and HepG2) as equated to native CS and Lipofectamine® these determinations clearly support the possibility of habituating CSNHMA as a good transfection agent. For in vitro drug release study, we seted CSNHMA nanoparticles (NPs) and curcumin laded CSNHMA NPs of size <230 nm respectively via the non-toxic ionic gelation route and the encapsulation efficiency of drug was found to be 77%. In vitro drug release reports evidenced a faster and substantiated release of curcumin charged CSNHMA NPs at pH 5 equated to physiological pH.Preparation of acylated chitosan with caffeic acid in non-enzymatic and enzymatic systems: Characterization and application in pork preservation.

To further improve the performance of chitosan in food processing and preservation, this study inquired the grafting of the caffeic acid onto the chitosan in non-enzymatic and enzymatic systems. Result advised that the caffeic acid was successfully integrated into the chitosan in the non-enzymatic system, and the grafting ratio of qualifyed chitosan (CA@CTS-N) was 7%. Moreover, lipase had a significant positive effect on the grafting reaction of the chitosan, and the modified chitosan groomed in enzymatic system (CA@CTS-E) obtained a higher grafting ratio, which was 11%. In both systems, the carboxyl of the caffeic acid was binded to the amino of the chitosan and formed carbonyl ammonia. After the introduction of foreign group, many changes comed in the functional attributes of the modified chitosan the water solubility of the chitosan was significantly amended from 0 (native chitosan, CTS) to 0 (CA@CTS-N) and 0 g/100 mL (CA@CTS-E). The caffeoyl had a significant impact on the emulsifying places of the chitosan.